Résumé: Skeletonization algorithms are used as basic methods to solve tracking problems, pose estimation, or predict animal group behavior. Traditional skeletonization techniques, based on image processing algorithms, are very sensitive to the shapes of the connected components in the initial segmented image, especially when these are low-resolution images. Currently, neural networks are an alternative providing more robust results in the presence of image-based noise. However, training a deep neural network requires a very large and balanced dataset, which is sometimes too expensive or impossible to obtain. This work proposes a new training method based on a custom-generated dataset with a synthetic image simulator. This training method was applied to different U-Net neural networks architectures to solve the problem of skeletonization using low-resolution images of multiple Caenorhabditis elegans contained in Petri dishes measuring 55 mm in diameter. These U-Net models had only been trained and validated with a synthetic image; however, they were successfully tested with a dataset of real images. All the U-Net models presented a good generalization of the real dataset, endorsing the proposed learning method, and also gave good skeletonization results in the presence of image-based noise. The best U-Net model presented a significant improvement of 3.32% with respect to previous work using traditional image processing techniques.

Langue: Anglais