ملخص: SummaryBackground: Rutin, a natural flavone derivative, is known for its pharmacological properties. We have previously reported that this flavonol exerted a potent inhibitory effect on respiratory burst of fMet-Leu-Phe-stimulated neutrophils, as well as on phosphoinositide 3-kinase [gamma] activity in a cell free system. In the present study, the anti-inflammatory effect of rutin was investigated in vivo and in vitro. Methods: rutin or aspirin (100 mg/kg, body weight) were given orally to rats 1 hour before paw oedema induction, using [lambda]-carrageenan 1%. The rat paw volume was measured by mean of plethysmometer, initially and during 6 hours. The chemotaxis of neutrophils towards 10-7 M fMet-Leu-Phe was performed using 48-well chemotaxis chamber. Neutrophils that migrated through 5 [mu]m pore size polycarbonate filter, in presence or in absence of rutin, were counted microscopically. Elastase exocytosis of either phorbol 12-myristate 13-acetate or fMet-Leu-Phe/cytochalasin B-stimulated neutrophils was assessed in absence or in presence of rutin using the synthetic substrate N-Suc-Ala-Ala-Ala-p-nitroanilide. The absorbance of released p-nitroaniline was measured at 405 nm using microplate reader. Results: The maximal swelling in placebo group was observed at 5 hours, after [lambda]-carrageenan injection. Oral administration of rutin reduced rat paw swelling starting 2 hours after [lambda]-carrageenan injection. Rutin reduced significantly (p Conclusion: The present study revealed that rutin possesses anti-inflammatory properties.

لغة: إنجليزية