Modification of the biosynthesis of rape lipid molecular species by heat shock
مقال من تأليف: Aid, Fatiha ; Mazliak, Paul ; Kesri-Benhassaine, Ghouziel ; Demandre, Chantal ;
ملخص: Rape, Brassica napus is a C16:3 plant, the leaves of which contain mainly ôprokaryotico; molecular species+ of monogalactosyldiacylglycerol (MGDG) (18 : 3/16 : 3, 76 mol%) and ôeukaryotico; molecular species of digalactosyldiacylglycerol (DGDG) (18 : 3/18 : 3, 70 mol%). Phosphatidylglycerol (PG) contains nearly exclusively ôprokaryotico; molecular species (18 : 3/16 : 1t, 18 : 3/16 : 0 and 18 : 2/16 : 1t), whereas phosphatidylcholine (PC) and phosphatidylethanolamine (PE) are exclusively formed by ôeukaryotico; (C18/C18, C16/C18) molecular species. A heat shock provoked a great decrease in [1-14C] acetate incorporation into rape leaf lipids, a decrease in the labelling of polyunsaturated fatty acids and an increase in the labelling of palmitic acid. After a heat shock, more saturated labelled molecular species (16 : 0/18 : 2, 16 : 0/18 : 3) appeared in PC and DGDG. The biosynthesis of prokaryotic MGDG molecular species was more inhibited by a heat shock than the biosynthesis of those of eukaryotic MGDG. Heat shock provoked an increase of 18 : 3/16 : 0 and 18 : 2/16 : 0 MGDG molecular species; thus, the chloroplastic palmitoyl MGDG desaturase could be affected. %
لغة:
إنجليزية